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詳細介紹
鏈霉親合素Streptavidin and Conjugates 每mg蛋白質結合17 to 24μg 生物素
品牌：上海阿拉丁 Aladdin
產(chǎn)品編號 ：S103034
CAS號： 9013-20-1
MDL號 MFCD00082035
鏈霉親合素,每mg蛋白質結合17 to 24μg 生物素,上海阿拉丁產(chǎn)品信息：
屬性
| 存貯條件 | -20°C 保存 |
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描述
| 別名 | 抗生物素蛋白鏈菌素，鏈親合素;Streptavidin 來源于阿維丁鏈霉菌;Streptavidin from Streptomyces avidinii;Streptavidin and Conjugates |
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| 產(chǎn)品介紹 | Streptavidin and Conjugates include streptavidin in a purified form, conjugated to enzymes or fluorophores or activated for crosslinking to sulfhydryl or carbonyl groups. These purified and conjugated forms of recombinant streptavidin protein enable detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western blotting, ELISA, immunohistochemistry and fluorescence imaging. Purified streptavidin and the streptavidin-HRP conjugates (peroxidase-conjugated streptavidin) are supplied lyophilized in phosphate-based buffers for immediate reconstitution with water. Alkaline Phosphatase conjugates are supplied as a liquid in a Tris-based buffer. |
| 用途 | Applications for enzyme-labeled (HRP, AP) streptavidin: Enzyme immunoassays (EIA), including enzyme-linked immunsorbent assays (ELISA) with biotinylated antibodies Cell and tissue staining (for light microscopy) Western blotting (WB) and other membrane-based biotin detection Applications for fluorescent (FITC, TRITC or Texas Red* Fluor-labeled) streptavidin: Immunofluorescent assays (IFA) Tissue staining (for fluorescence microscopy) Blot immunostaining Cell staining (incl. fluorescent activated cell sorting) |
| 生化機理 | reptavidin is biotin-binding protein that was originally isolated from Streptomyces avidinii. In contrast to avidin, streptavidin has no carbohydrate and has a mildly acidic pI of 5. OursStreptavidin products use a recombinant form of streptavidin having a mass of 53,000 daltons and a near-neutral pI. Streptavidin is a tetrameric protein, with each subunit binding one molecule of biotin with affinity similar to that of avidin. Guanidinium chloride will dissociate avidin and streptavidin into subunits, but streptavidin is more resistant to dissociation. |
| 包裝 | S103034-1mg,S103034-5mg,S103034-25mg in glass bottle |
| 備注 | 單位定義：1單位結合1.0 UG 生物素. 方法描述見文獻 GREEN N.M., METHODS IN ENZYMOLOGY, 18, 418 (1970). 來源：Streptomyces avidinii、
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| 個人防護設備 | Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter |
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| WGK德國 | 3 |