中文摘要：

腱?骨損傷患者因天然結(jié)構(gòu)破壞導(dǎo)致運(yùn)動(dòng)功能受限，阻礙損傷恢復(fù)。傳統(tǒng)生物材料側(cè)重于增強(qiáng)肌腱 / 骨的再生能力以重建天然結(jié)構(gòu)，但由于忽視免疫微環(huán)境且缺乏多組織再生功能，往往難以獲得理想的修復(fù)效果。本研究將硅酸錳（MS）納米顆粒與肌腱 / 骨相關(guān)細(xì)胞復(fù)合，制備出可用于腱?骨一體化再生的免疫調(diào)節(jié)型多細(xì)胞支架。

值得注意的是，通過(guò)整合仿生細(xì)胞排布與硅酸錳納米顆粒，該多細(xì)胞支架展現(xiàn)出多重生物活性。此外，硅酸錳納米顆?？赏ㄟ^(guò)調(diào)控巨噬細(xì)胞，促進(jìn)支架內(nèi)多細(xì)胞的特異性分化；該效應(yīng)主要源于錳離子誘導(dǎo)巨噬細(xì)胞分泌前列腺素 E2（PGE2）。多項(xiàng)動(dòng)物實(shí)驗(yàn)結(jié)果證實(shí)，該支架能夠在腱?骨界面實(shí)現(xiàn)免疫調(diào)節(jié)、一體化再生及運(yùn)動(dòng)功能恢復(fù)。

綜上，基于無(wú)機(jī)生物材料構(gòu)建的多細(xì)胞支架，為軟 / 硬組織界面的免疫調(diào)控與一體化再生提供了創(chuàng)新思路。

英文摘要：

Limited motor activity due to the loss of natural structure impedes recovery in patients suffering from tendon-to-bone injury. Conventional biomaterials focus on strengthening the regenerative ability of tendons/bones to restore natural structure. However, owing to ignoring the immune environment and lack of multi-tissue regenerative function, satisfactory outcomes remain elusive. Here, combined manganese silicate (MS) nanoparticles with tendon/bone-related cells, the immunomodulatory multicellular scaffolds were fabricated for integrated regeneration of tendon-to-bone. Notably, by integrating biomimetic cellular distribution and MS nanoparticles, the multicellular scaffolds exhibited diverse bioactivities. Moreover, MS nanoparticles enhanced the specific differentiation of multicellular scaffolds via regulating macrophages, which was mainly attributed to the secretion of PGE2 in macrophages induced by Mn ions. Furthermore, three animal results indicated that the scaffolds achieved immunomodulation, integrated regeneration, and function recovery at tendon-to-bone interfaces. Thus, the multicellular scaffolds based on inorganic biomaterials offer an innovative concept for immunomodulation and integrated regeneration of soft/hard tissue interfaces.

論文信息：

論文題目：Immunomodulatory multicellular scaffolds for tendon-to-bone regeneration

期刊名稱(chēng)：Science Advances

時(shí)間期卷：Vol 10, Issue 10(2024)

在線時(shí)間：2024年3月8日

DOI: 10.1126/sciadv.adk6610

產(chǎn)品信息：

貨號(hào)：CP-005-005

規(guī)格：5ml+5ml

品牌：Liposoma

產(chǎn)地：荷蘭

名稱(chēng)：Clodronate Liposomes&Control Liposomes

辦事處：靶點(diǎn)科技

Clodronate Liposomes氯膦酸鹽脂質(zhì)體在肌腱 / 骨相關(guān)細(xì)胞復(fù)合模型種清除巨噬細(xì)胞。荷蘭Liposoma巨噬細(xì)胞清除劑ClodronateLiposomes見(jiàn)刊于Science Advances：用于腱?骨再生的免疫調(diào)節(jié)型多細(xì)胞支架。

Liposoma巨噬細(xì)胞清除劑Clodronate Liposomes氯膦酸二鈉脂質(zhì)體清除巨噬細(xì)胞的材料和方法：

Depletion of AMs

To investigate the effect of macrophage depletion on enhanced regenerative outcomes induced by the scaffolds containing MS nanoparticles, the macrophage depletion model of Sprague-Dawley rats was created according to a previous report (57). Clodronate liposomes (LIPOSOMA, Netherlands) were applied to deplete macrophages. First, the Sprague-Dawley rats (male, 6 weeks old) were randomly divided into two groups: one group was treated with liposome-encapsulated PBS (GelMA-cells-MS+PBS) and another group was treated with clodronate liposomes (GelMA-cells-MS+clodronate). All of the rats received injections on days 1 and 3 before the implantation of scaffolds. After being shaved, the backs of the rats were sterilized with iodine solution. Then, a subcutaneous cavity was created on the side of the back, enabling the insertion of a scaffold. The skin incision was closed by 4-0 Ethibond sutures. Subsequently, sterile liposomal clodronate or liposome-encapsulated PBS (50 mg/kg) was locally injected in the cavity every 3 days from the first postoperative day. After 2 weeks of operation, the rats were euthanized and the scaffolds were collected. When analyzing the above results, the researchers were blinded to the grouping of animals.

巨噬細(xì)胞清除材料和方法文獻(xiàn)截圖：用于腱?骨再生的免疫調(diào)節(jié)型多細(xì)胞支架